WebThe Ion S5 Calibration Standard can be used in conjunction with the Ion 520™ and Ion 530™ workflows for de novo research sequencing applications to allow for greater … WebThe library was sequenced on an Ion S5TM XL platform. Library strategy: OTHER: Library source: genomic: Library selection: other: Instrument model: Ion Torrent S5 XL : Data processing: Single-end reads was assigned to samples based on their unique barcode and truncated by cutting off the barcode and primer sequence.
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WebApplications Ion S5TMand Ion S5TMXL Systems • Simplest NGS workflow for targeted sequencing - <15mins to set up a sequencing run and <45mins total hands-on time from DNA to data with Ion Chef™ System • Fastest run time – as little as 3.5 hours from sequence to BAM files. Web• Ion S5™ Sequencing Reagents (Part no. A27768; 2 boxes per kit) The Ion 540™ Kit – Chef contains template preparation reagents sufficient for 8 reactions for automated … la kasbah hotel tunisia
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Web10 aug. 2024 · Then, Ion S5TM XL platform was used for library sequencing. Data Analysis The V4 region was amplified through custom barcoded primers. Raw reads were filtrated through Cutadapt software, and then the reads were compared with the Silva database for removing the chimera sequences. Finally, the clean reads were obtained. WebMitoSOX™ Red reagent is a fluorogenic dye specifically targeted to mitochondria in live cells, and its oxidation by superoxide produces red fluorescence. Briefly, 2.5 µM MitoSOX were added to each well, and incubated for 30 min at 37 °C, protected from light. WebThe libraries were sequenced using an Ion S5TM XL platform or an Illumina Hiseq2500 platform, and 600 bp single-end reads or 250 bp paired-end reads were generated. (ii) Pacbio library preparation and sequencing. Nearly complete 16S rRNA genes were amplified using primers 27F/1492R [27]. jemts gräv