Diacetylchitobiose deacetylase
WebSo far chitin deacetylase from fungi and insects, chitooligosaccharide deacetylase (NodB) from Rhizobium, and bacterial GlcNAc6P deacetylase have been known as catalytically … WebJan 24, 2024 · Diacetylchitobiose deacetylase (EC 3.5.1.105) belongs to the carbohydrate esterase family 14, it is the key enzyme that catalyzes the hydrolysis of N-acetyl groups …
Diacetylchitobiose deacetylase
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WebOct 1, 2024 · Directed evolution of diacetylchitobiose deacetylase via high-throughput droplet sorting with a novel, bacteria-based biosensor Request PDF Directed evolution … WebThe hyperthermophilic archaeon Thermococcus kodakaraensis KOD1 possesses chitinase (Tk-ChiA) and exo-β-d-glucosaminidase (Tk-GlmA) for chitin degradation; the former produces diacetylchitobiose (GlcNAc2) from chitin, and the latter hydrolyzes chitobiose (GlcN2) to glucosamine (GlcN). To identify the enzyme that physiologically …
WeblationofGlcNActoformGlcN,diacetylchitobiose(or chitin disaccharide) deacetylase, and N-acetyl-D-glucosamine-6-phosphate (GlcNAc-6P) deacetylase. Diacetylchitobiosedeacetylase(EC3.5.1.105)belongs to the carbohydrate esterase family 14, it is the key enzyme that catalyzes the hydrolysis of N-acetyl groups at the non … WebJun 28, 2024 · Diacetylchitobiose deacetylase (Dac) from Pyrococcus horikoshii can realize the one-step production of glucosamine (GlcN). The efficient expression and secretion of Dac play a central role in the ...
WebDec 1, 2024 · Diacetylchitobiose deacetylase (Dac) from Pyrococcus horikoshii can realize the one-step production of glucosamine (GlcN). The efficient expression and secretion of Dac play a central role in the green production of GlcN. In this study, Bacillus subtilis WB600 was used as the expression host. Firstly, we screened 12 signal … WebConcerted Action of Diacetylchitobiose Deacetylase and Exo- -D-glucosaminidase in a Novel Chitinolytic Pathway in the Hyperthermophilic Archaeon Thermococcus kodakaraensis KOD1* Received for publication, December 26, 2003, and in revised form, May 3, 2004 Published, JBC Papers in Press, May 10, 2004, DOI 10.1074/jbc.M314187200
WebJun 16, 2012 · N,N'-diacetylchitobiose deacetylase (Dac) belongs to the CE-14 family and plays a role in the chitinolytic pathway in archaea by deacetylating N,N'-diacetylchitobiose (GlcNAc2), which is the end ...
WebAug 24, 2024 · This deacetylase can also use diacetylchitobiose-6-phosphate as a substrate to produce monoacetylchitobiose-6-phosphate, which is the inducer and … 高ナトリウム血症WebAug 1, 2024 · Diacetylchitobiose deacetylase (Dac) from Pyrococcus horikoshii can realize the one-step production of glucosamine (GlcN). The efficient expression and secretion of Dac play a central role in the ... tartan spartan setWebJul 1, 2013 · The purified Pyrococcus Dacs were active and thermostable up to 85 °C. The crystal structures of Ph-Dac and Pf-Dac were determined at resolutions of 2.0 Å and 1.54 Å, respectively. The Pyrococcus Dac forms a hexamer composed of two trimers. These Dacs are characterized by an intermolecular cleft, which is formed by two polypeptides in the ... tartan spartan searing plasmaWebOct 28, 2024 · Deacetylase from the extremophile Pyrococcus chitonophagus has been analyzed by X-ray crystallography, small-angle X-ray scattering, differential scanning calorimetry, isothermal titration calorimetry and NMR to determine its structure, thermodynamics and enzymatic properties ... tartan spartan pyroWebDec 1, 2024 · Diacetylchitobiose deacetylase Expression element optimization Cell factory Bacillus subtilis Protein secretion 1. Introduction Glucosamine (GlcN) plays an … 高ナトリウム血症 原因WebSelect search scope, currently: articles+ all catalog, articles, website, & more in one search; catalog books, media & more in the Stanford Libraries' collections; articles+ journal articles & other e-resources 高ナトリウム血症 心電図WebIn this study, semi-rational design based on site-directed saturation mutagenesis and surface charge modification was used to improve the catalytic efficiency of the … 高ナトリウム血症 ガイドライン