Dh5 alpha subcloning efficiency
WebDec 13, 2011 · E. coli. (Subcloning Efficiency)? The properties of this strain that contribute to its usefulness as a cloning strain are described below. The genotypes underlying these properties appear in parentheses. Blue/White Screening: (Φ80 Δ (lacZ)M15 makes the omega-fragment of β-galactosidase (β-gal); (argF-lacZ) deletes the β-gal gene on the ... Web5 Using DH5α-T1R as a Transient Host Introduction DH5α-T1R competent E. coli support replication of M13mp vectors, but it does not support plaque formation. Plating on a lawn of E. coli containing the F plasmid will allow plaque formation. Before Starting You will need the following reagents: • Log-phase E. coli containing the F plasmid (e.g. DH5α-FT™, …
Dh5 alpha subcloning efficiency
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WebNEB 5-alpha Competent E. coli (High Efficiency) C2987: 6 x 0.2ml: Subcloning Efficiency ™ DH5α ™ Competent Cells* ThermoFisher (Invitrogen) 18265-017: 4 x 0.5ml: NEB 5-alpha Competent E. coli (Subcloning Efficiency) C2988: 6 x 0.4ml: MAX Efficiency ® DH5αF'IQ ™ Competent Cells* ThermoFisher (Invitrogen) 18288-019: 5 x 0.2ml WebYour Price. C2988J. 6 x 0.4 ml. $106.00. $95.40. *On-line ordering is for Canadian customers only. Web pricing is applicable only to orders placed online at www.neb.ca. NEB 5-alpha Competent E. coli is a derivative of …
WebE. coli. (High Efficiency) NEB 5-alpha Competent E. coli is a derivative of the popular DH5α. It is T1 phage resistant and endA deficient for high-quality plasmid preparations. High efficiency strain ideal for a wide variety of applications. Available in a wide variety of sizes, including single-use vials, 200 μl vials, 96- and 384- well ... WebDh5-Alpha Competent E. coli 320 Harbor Way South San Francisco, CA 94080 Phone: 1 (888) MCLAB-88 Fax: 1 (650) 871-8796 www.mclab.com ... (0.01 μg/ml) is provided as a control to determine the transformation efficiency. To obtain maximum transformation efficiency, the experimental DNA must be free of phenol, ethanol, protein
WebIntact Genomics 5-alpha electrocompetent E. coli cells are suitable for high efficiency transformation in a wide variety of routine applications such as plasmid isolation, cloning, and subcloning. Mutations in endA1 and recA1 ensure increased plasmid yield and improved plasmid quality. WebDec 13, 2011 · E. coli. (Subcloning Efficiency)? The properties of this strain that contribute to its usefulness as a cloning strain are described below. The genotypes underlying …
WebSubcloning Efficiency™ DH5α™ Competent Cells are tested for transformation efficiency using the pUC19 control DNA supplied with the kit and using the protocol on page 2. …
WebMar 16, 2024 · Economical for routine subcloning procedures or applications where starting DNA is not limiting. Brand: Invitrogen™ 18265017. Additional Details : Weight : 0.05000kg. Product Code. 11583117. 83.25 € / 2mL. Quantity. Add to basket. Includes: Subcloning Efficiency DH5α Competent Cells: 4 vials, 500μL each (total of 2mL), pUC19 DNA … classic scottish startersWebChemically competent E. coli cells suitable for subcloning efficiency transformations, such as plasmid transformation or routine subcloning (e.g,. inserting a 1 kb fragment into a 2 kb vector). The efficiency of these cells is not sufficiently high for most other cloning applications. For that, we recommend NEB® 5-alpha Competent E. coli (High … download pbs kids games for freeWebSubcloning Efficiency™ DH5α™ Chemically Competent E. coli Catalog no. 18265-017 General Information and Kit Contents Shipping and Storage The kit is shipped on dry ice. … download pbs kids freeWebDH5 is a well-known, versatile strain that can be used in many everyday cloning applications. In addition to supporting blue/white screening, recA1 and endA1 mutations in DH5 increase insert stability and imp ... download pbs kids video akp modWebDH5-alpha Comp Cells SUBCLONING 500 ul # 18265-017: each: Life Technologies: 57648: DH5-alphaCompCells MAX Efficiency 200ul #18258012: each: Life Technologies: 57649: BL21 STAR (DE3) OneShot CompCells 50 ul #C6010-03: ... NEB 5-alpha Competent E.Coli 20 x .05 ml # C2987H: box: download p. b. s. kids gamesWebStandard efficiency (propagation) Subcloning Efficiency™ DH5α™9 (1 x 106 cfu/µg) gDNA or library production One Shot™ OmniMAX 2 T1R (>20 kb) ElectroMAX ™ DH10BOne Shot (>20 kb) ElectroMAX ™ DH10B T1R (>20 kb) MegaX DH10B ™ T1R Electrocomp ™8 (>100 kb) High transformation efficiency (cloning) One Shot ™ … classic scotcheroo recipeWebSubcloning Efficiency™ DH5α™ Chemically Competent E. coli Catalog no. 18265-017 General Information and Kit Contents Shipping and Storage The kit is shipped on dry ice. Upon receipt, store the kit at -70°C. Do not store in liquid nitrogen. Kit Contents The kit contains the following reagents. Reagent Composition Amount classics course