site stats

Cdh5 cre mouse

WebApr 14, 2024 · The following mouse strains were used (all on a C57BL/6 background): Cdh5 CreERT2:ZsGreen l/s/l mice (control mice; TgCdh5-Cre/ERT2 1Rha) and Cdh5 CreERT2:Dnmt1 fl/fl:ZsGreen l/s/l mice (Dnmt1 ... Web033055 B6.Cg-Tg(Cdh5-cre)1Spe/J VEC-cre transgenic mice express Cre recombinase under the control of a Cdh5 promoter with greater efficiency than other similar lines. They may have applications related to the study of hematopoietic stem cell lineages derived from vascular endothelial cells.

Vacation rentals in Fawn Creek Township - Airbnb

Web017968 B6;129-Tg(Cdh5-cre)1Spe/J These VEC-cre transgenic mice express Cre recombinase under the control of a Cdh5 promoter with greater efficiency than other … Please use this form to enter mouse-related technical questions, comments, … Updated features: Recombinase (cre) structure search enhancements, … WebOct 3, 2024 · Disclosed are methods and compositions for treating cardiovascular diseases including cardiomyopathy and heart failure. Particularly disclosed are methods and compositions that utilize or comprise inhibitors of hypoxia-inducible factor (HIF)-2α or agonists/inducers of HIF prolyl hydroxylase domain-2 (PHD2) signaling for treating … bambam tree https://nmcfd.com

National Center for Biotechnology Information

WebSep 9, 2024 · Whereas Tg(Cdh5-cre/ERT2)1Rha is present in five copies 86, the independently generated Tg ... Thanos, A. et al. Evidence for baseline retinal pigment epithelium pathology in the Trp1-Cre mouse. WebJul 9, 2024 · Here, we describe the development of an autochthonous mouse model of angiosarcoma driven by loss of p53 in VE-cadherin-expressing endothelial cells. Using Cdh5-Cre to drive recombination in adult endothelial cells, mice developed angiosarcomas with 100% penetrance upon homozygous deletion of Trp53 with a median lifespan of 325 … WebMar 30, 2024 · Characterization of the Hrh2 flox/flox, cdh5−Cre mouse. The #69 ES cell clone was then selected for blastocyst microinjection to generate the founder generation Hrh2 floxneo mice (F0), and deletion of Neo in the F1 (Hrh2 flox/+) mice was accomplished by crossing F0 male mice with WT females. armidian karyatama tbk annual report

Endothelial-Specific Cre Mouse Models Arteriosclerosis, …

Category:The Cdh5-CreERT2 transgene causes conditional Shb …

Tags:Cdh5 cre mouse

Cdh5 cre mouse

Vacation rentals in Fawn Creek Township - Airbnb

WebNational Center for Biotechnology Information WebThe Cdh5(PAC)-CreERT2 Mouse was developed in the laboratory of Ralf Adams at the London Research Institute. The model was generated by microinjecting a transgene containing a genomic Cdh5(PAC) promoter …

Cdh5 cre mouse

Did you know?

WebApr 11, 2024 · Mouse livers were harvested and fixed in a 4% formaldehyde solution. Paraffin-embedded liver tissues were sectioned into 8-μm-thick pieces, and OCT-embedded liver tissues were cut into 8-μm-thick sections. ... (S1pr2 ECTg) by intercrossing the Cdh5-Cre ERT2 line with S1pr2 Tg/wt mice to overexpress S1pr2 in LSECs (Figure 4A). RT … WebMar 28, 2024 · The tamoxifen-inducible cre/ERT2 sequence was inserted into a PAC (P1 artificial chromosome) containing the VE-cadherin (Cdh5) gene to generate the …

Web006137 B6.FVB-Tg(Cdh5-cre)7Mlia/J Cre expression in these transgenic mice can be seen in the embryo and in adult endothelium of developing and quiescent vessels, as well as … WebAbstract. To introduce temporal control in genetic experiments targeting the endothelium, we established a mouse line expressing tamoxifen-inducible Cre-recombinase (Cre-ERT2) under the regulation of the vascular endothelial cadherin promoter (VECad). Specificity and efficiency of Cre activity was documented by crossing VECad-Cre-ERT2 with the ...

WebJun 12, 2014 · In addition, although duplex PCR of WBC DNA clearly shows that Cdh5 (Spe)-Cre is more efficient than Cdh5 (Mlia)-Cre, the relative efficiency of Tek-Cre is less clear. It becomes obvious from singleplex F8 F -specific PCR results that Tek-Cre is in fact virtually 100% efficient in WBCs, whereas a significant amount of unrecombined F8 F … WebMay 17, 2024 · The Cdh5-CreERT2 transgene causes conditional Shb gene deletion in hematopoietic cells with consequences for immune cell responses to tumors. The …

http://www.informatics.jax.org/recombinase/specificity?id=MGI:3848982&system=cardiovascular+system

WebCdh5-Cre/ERT2 alleles are the most commonly used inducible EC-specific Cre mice. There are 3 different alleles that use regions of the Cdh5 … armida salsiah alisjahbanaWebConstitutively Active Pan-Endothelial Cre Mouse Models In constitutively active pan-endothelial Cre transgenic mice, Cre recombinase activity is driven by enhancer and … armi da softair san marinoWebTg (Cdh5-cre/ERT2)#Ykub. Mutation details : The transgene was constructed from BAC clone RP23-453P1 to use the 200.3 kb mouse Cdh5 promoter to drive expression of an inducible cre recombinase gene (cre/ERT2). The pound symbol (#) is used when no line is specified and/or lines are pooled. bambam transit loveWebWant a minute-by-minute forecast for Fawn-Creek, Kansas? MSN Weather tracks it all, from precipitation predictions to severe weather warnings, air quality updates, and even … armi dean kamenWebBed & Board 2-bedroom 1-bath Updated Bungalow. 1 hour to Tulsa, OK 50 minutes to Pioneer Woman You will be close to everything when you stay at this centrally-located … armidian karyatama tbkhttp://www.informatics.jax.org/recombinase/specificity?id=MGI:3848982&system=cardiovascular+system armi da softair usateWebGeneration of Cdh5 Cre - and Tie1 Cre-driven mouse models to genetically delete Twist1 or Snai1 in endothelial cells. Fig. S16. Mice with conditional deletion of Twist1 or Snai1 in Cdh5-expressing endothelial cells are … bam bam toys murwillumbah